Silane-based modified papers and their extractive phase roles in a
microfluidic platform
Mahin Hashemi Hedeshi , Omid Rezvani, Habib Bagheri
Analytica Chimica Acta 1128 (2020) 31-41
doi: 10.1016/j.aca.2020.05.069
Herein, some (modified) paperebased substrates were prepared and utilized as extractive phases in a microfluidic device and their extraction performances examined for analytes with different polarities.
Reagents including hexadecyltrimethoxysilane (HDTMS), phenyltrimethoxysilane (PTES), (3-aminopropyl) triethoxysilane (APTES) and 3-(2,3-epoxypropoxy) propyltrimethoxysilane (EPPTMOS) were
implemented for the modification process. Due to the induction of different silane functional groups, it
was anticipated to have various interactions for the tested analytes. Eventually, the prepared paper
sheets were used as extractive phases for solidephase extraction within a microfluidic system. The
microfluidic setup not only improves the mechanical stability of the paperebased phases but also facilitated the mass transfer process and decreases the energy consumption. The selected analytes
contained antidepressant drugs, organophosphates and triazine toxins, having different structures and
polarities. Afterward, the existing interactions between the paperebased sorbents and the selected
analytes were investigated by Principal Component Analysis (PCA) and Hierarchical Clustering Analysis
(HCA) for data analysis. According to the obtained data, the PTES modified surface paper was selected for
quantitative investigation and optimization. The major parameters associated with the extraction performance were studied. A linear range was obtained for amitriptyline and trimipramine, 0.05-200 mg L-1, and the calibration graph for clomipramine was linear in the range of 0.02-200 mg L-1. The
employment of microfluidic device in conjunction with gas chromatography-mass spectrometry led to
the limits of detections of 0.005-0.01 mg L-1 for the antidepressant drugs. The relative recoveries for the
tested drugs in urine samples were in the range of 95-103%.